These species are used for food, flavourings, cosmetics, and for medicinal purposes. Improvements of doubled haploid production protocol for white. Get now the soft documents of this book doubled haploid production in crop plants. Doublehaploid induction speeds up plantbreeding process. Haploid and doubled haploid plants are selected by a largescale screening based on a combination of the phenotype with the use of molecular methods combined with flow cytometry techniques to identify haploid and doubled. Maternal haploids are preferentially induced by cenh3. Nov 30, 2003 buy doubled haploid production in crop plants. A manualfrom springer details that our company offer, you may not be so baffled to be here and also to be participant. Development and characterization of elite doubled haploid.
Haploids and doubled haploids in plant breeding intechopen. To test if haploid inducer lines can be engineered into crops, cenh3 and cenh3. Haploid plants are intensively utilized for investigation and improvement of many agricultural crops. Improvements of doubled haploid production protocol for. Albinism is a major limitation in the production of doubled haploid plants in cereals. They are smaller and exhibit a lower plant vigor compared to donor plants and are sterile due to the inability of their chromosomes to pair during meiosis. Wo2017200386a1 method for the production of haploid and. Thus, in a diploid sporophytic 2 n species the haploid could also be called monoploid xas. Comparison of different androgenesis protocols for doubled. The production of doubled haploid dh barley hordeum vulgare l.
Frontiers doubled haploid production from spanish onion. Here we present the first highthroughput screen to identify. Since the construction of genetic maps using molecular markers requires the development of segregating doubled haploid. Doubled haploid production heartland plant innovations. Molecular characterization of mutant germplasm a manual. However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also. If you wish more information about this publication, then please email to p. Production of doubled haploids dhs is an important methodology to speed the process of breeding and development of mapping populations in crops. Welcome to the how to use doubledhaploids to improve winter wheat webinar todays presenters. Nov 01, 2010 a scientific explanation of the doubled haploid dh technique, including a step by step guide for the creation of a dh line. Haploids from isolated and in vitro cultured anthers anther culture is often the method of choice for doubled haploid production in crop plants 29.
Conservation of mtl in cereals may enable the development of intraspecific in vivo haploid inducer lines in crop plants to accelerate plant breeding. A critical component of this method depends on chromosome doubling, which is traditionally achieved by in vivo treatment of seedlings with colchicine. Efficiency of wheat dh production nb of embryo100 florets. The present study represents the first report of the production of haploid and doubled haploid chrysanthemum plants derived from the in vitro culture of nonfertilized ovules, morphological traits were changed significantly in the haploid plants. From springer doubled haploid production in crop plants. Embryolike structure els formation was significantly affected by.
Read this article to learn about the production of haploid plants. An improved protocol for carrot haploid and doubled haploid. Impacts efficient production of doubled haploid lines could cut time for development of onion inbred lines by 75 percent or more, accelerating the introgression of resistance and other desirable traits into materials adapted to ny state. Maluszynski 2003 doubled haploid production in crop plants. Improved efficiency of doubled haploid generation in. Validation of doubled haploid plants by enzymatic mismatch. Doubled haploid production in crop plants springerlink. A wide range of methods has been developed to produce doubled haploid dh plants and recent advances promise efficient dh production in otherwise recalcitrant species. However, there have been several approaches to develop doubled haploids in many crops or plant species.
Methods to obtain dh triticale lines using anther or isolated microspore culture can be found in the other chapters of this manual. The genetic upgradation of crops through conventional breeding approaches require. The attaining homozygous line in the normal breeding was crucial in order to develop hybrids. Plant haploid research began with the discovery that sporophytes can be produced in higher plants carrying the gametic chromosome number n instead of 2n and that their chromosome number can subsequently be doubled up by colchicine treatment. Oil palm is the worlds most productive oilfood crop despite yielding well below its theoretical maximum. Comparison of different androgenesis protocols for doubled haploid plant production in ornamental pepper capsicum annuum l. Pdf download doubled haploid production in crop plants. Colchicine treatment results in double haploid plants with 100% homozygosity. Corn is a prime example of what can happen when scientists use the doubled haploid. The procedure for dh production includes two major steps. But most techniques for the production of haploids if a technique exists at all for a given crop require advanced tissue culture facilities and very skilled technicians. The use of doubled haploids as parents for f 1 variety production fully exploits this phenomenon and has enabled substantial yield improvements in several crops 2,3.
In addition, doubling the chromosome number of a haploid to produce a doubled haploid results in a completely homozygous plant. Haploid is the general term for plants sporophytes that contain the gametic chromosome number n. Based on the doubled haploid production in crop plants. Doubled haploids as a material for biotechnological. Isolated microspore culture techniques and recent progress. Doubled haploid production in crop plants a manual. Mar 10, 2015 colchicine treatment concentration mgl colchicine treatment duration h number of regenerated plants number of doubled haploid plants 0 control 0 53 0 125 12 49 10 24 37 9 36 18 8 250 12 41 24 42 27 36 15 9 500 12 17 7 24 14 8 36 9 6 12 0 0 24 0 0 36 0 0 18. Additionally, methods for making such plants as well as methods for producing haploid or doubled haploid plants using such plants are disclosed. Published doubled haploid protocols in plant species.
The crops covered include barley, wheat, maize, rice, triticale, rye, oats, durum wheat, timothy grass phleum pratense, ryegrass lolium, rape, broccoli, tobacco, potato, flaxlinseed. Isolated microspore culture techniques for haploid and doubled haploid plant production an isolated microspore culture provides an excellent system for the study of microspore induction and embryogenesis and can produce doubled haploid dh plants, which are used to accelerate plantbreeding programs. Moreover, isolated microspore cultures have several advantages over anther culture, wherein presence. Cells at meristematic stage are sensitive to the treatment. Role of doubled haploids in vegetable crop improvement. Doubled haploids in crop improvement crops plant breeding. Msap profiling suggested that alterations in cytosine methylation occurred as a result of both the.
Doubled haploidy and induced mutation doubled haploidy and induced mutation szarejko, i forster, b. Characterization of in vitro haploid and doubled haploid. That is where the real value would be, as it could really make a breakthrough in the breeding of these crops. This manual presents a set of protocols for the production of doubled haploid plants in 22 major crops species including 4 tree species, and includes protocols from different germplasm of the same species. Haploids are unique plants and can provide researchers with genetic information not possible with normal diploid individuals. Among the most promising emerging technologies is the production of doubled haploids genetically pure plant lines that offer a quick.
Doubled haploid production in nutraceutical species. Gynogenesis and doubled haploid production from unpollinated. A manual from springer the production of doubled haploids has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. Doubled haploid production in crop plants a manual, plant. A doubled haploid dh is a genotype formed when haploid cells undergo chromosome doubling. Mar 31, 2016 doubled haploid plants are invaluable breeding tools but many crop species are recalcitrant to available haploid induction techniques. Everyday low prices and free delivery on eligible orders. Pdf haploids and doubled haploids in plant breeding. Today, advanced plant breeding technologies enable far more significant improvements in far less time. Success of early f 1 hybrid maize varieties exemplifies the advantages of heterosis. Oct 05, 2006 doubled haploid production in nutraceutical species.
Artificial production of doubled haploids is important in plant breeding haploid cells are produced from pollen or egg cells or from other cells of the gametophyte, then by induced or spontaneous chromosome doubling, a doubled haploid cell is produced, which can be grown into a doubled haploid plant. In research, doubled haploid lines have been used for structural and functional genomics, markertrait association studies, and molecular cytogenetics chang and coe, 2009. Everything you need to know about doubled haploids in crop improvement. Introduction oil seeds are important source of edible oil production and are cultivated on a large scale in the world. In this study we investigated the applicability of an in vitro approach for chromosome doubling based on microspore culture. There are two approaches for the production of haploid plants. Haploid induction via manipulation of cenh3 university. Maize doubled haploid dh operations manager the international maize and wheat improvement center cimmyt seeks a resultsoriented and highly motivated operations manager for establishing and successfully operating the maize doubled haploid dh facility in africa. Prepared by the joint faoiaea programme of nuclear techniques in food and agriculture, plant breeding and genetics laboratory, seibersdorf, 2014. Increased recovery of green doubled haploid plants from. Doubled haploid production in crop plants a manual m. Fortyfour protocols of doubled haploid production dh are presented in this manual.
This is the first manual presenting a set of prools for production of doubled haploids dh in 22 major crop plant species including 4 tree species. Novel technologies in doubled haploid line development. Doubled haploid production in crop plants request pdf. Following those initial discoveries, many studies have been conducted to develop haploids in brassica plants. The chromosome number could be doubled by chemical treatment i. Colchicine prevents cell division by inactivating the spindle mechanism.
It contains various protocols and approaches of dh production proven for different germplasm of the same species. A manualfrom springer as well as save it to be yours. Optimizing protocol for efficient microspore embryogenesis and doubled haploid development in different maturity groups of cauliflower b. Haploid and doubled haploid techniques in perennial. Since the cellular origin of the plants produced is not. Doubled haploidy and induced mutation, euphytica 10. Good aseptic techniques are required, but methods are generally simple and. In indica rice, success using the doubled haploid approach was demonstrated recently through the development of two varieties, satya krishna and phalguni, at central rice research institute. The second is deploying the technology to other crops that do not have any doubledhaploid production system, he says.
Ebook pdf download doubled haploid production in crop. Optimization of doubled haploid production in maize zea. Novel technologies in doubled haploid line development ren. They are related to at least 33 plant species, not counting protocols on production of dh from interspecific hybrids. Request pdf doubled haploid production in crop plants after many years of different experiments around the world on haploid production different methods have become available to the breeders. Scottish crop research institute, invergowrie, dundee, dd2 5da, scotland, uk. Doubled haploids refer to the diploid lines which are obtained by doubling the chromosome number of a haploid line by colchicine treatment. The production of doubled haploids has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. Ninetyfour doubled haploid dh lines derived from five crosses and their respective parents were sown in randomized complete blocks design with three replications in 200910 season. Pages 5358 in doubled haploid p roduction in crop plants. Wo2008114145a2 methods of producing haploid and doubled. The development of such elite lines has thus far been prevented by difficulties in generating homozygous parental types for f1 generation. Doubled haploids in genetics and plant breeding plant.
Anther culture for doubled haploid production in barley hordeum vulgare l. Each experimental unit consisted of a single row with a length of 3 m for each entry. Effects of colchicine concentration and duration treatment on regeneration and recovery of doubled haploid plants of oilseed rape. Method for the production of haploid and subsequent doubled haploid plants download pdf info. More particularly, but not exclusively, the method may be used for selecting haploid and doubled haploid oil palm plants. Doubled haploid production in crop plants a manual edited by m. Production of haploids and doubled haploids in oil palm bmc. In fact, under optimal conditions, doubled haploids dh have been. Dh plants obtained are being transferred to soil for evaluation and use in the cornell onion breeding program. Improvements in the production of doubled haploids in durum wheat. In recent years, wide hybridization between wheat and maize has become a main approach for haploid production in wheat. The effects of the genotypes of donor plants and three steps of culture media induction, differentiation, and regeneration media were evaluated in unpollinated ovary culture of cucumber cucumis sativus l. This maximum could be approached with the introduction of elite f1 varieties. Strategy for improvement of doubled haploid production in.
Seeds obtained from doubled haploid plants were harvested. It doesnt affect chromosome replication, but does prolong the time for mitosis. The ability to produce haploid plants is a tremendous asset in genetic and plant breeding studies. The importance of this in vitro process is the fact that the resulting haploid or doubled haploid dh embryo and plant can be used to shorten breeding programmes for production of new crop. The present study aims to generate elite doubled haploids from two elite indica hybrids rice crhr5 and crhr7. The hormone treatment was altered to use dicamba rather than 2,4d, which gave a twofold increase in the number of. This is the first manual presenting a set of protocols for production of doubled haploids dh in 22 major crop plant species including 4 tree species. They are related to at least 33 plant species, not counting protocols on production of dh from interspecific. Production of haploids and doubled haploids in oil palm.
Manual was prepared to stimulate the wider use of this technology for speeding and opening up new breeding possibilities for may crops including some woody species. Review of doubled haploid production in durum and common. The origin and homozygosity of diploid regenerants cannot be determined by chromosome counting or flow cytometry as they contain the same amount of nuclei and chromosomes numbers diao et al. Sep 23, 2017 the effects of the genotypes of donor plants and three steps of culture media induction, differentiation, and regeneration media were evaluated in unpollinated ovary culture of cucumber cucumis sativus l.
This strategy was outlined with the first dh crop variety and has led to hdh production systems being described for 250. An improved protocol for carrot haploid and doubled haploid plant production using induced parthenogenesis and ovule excision in vitro agnieszka kielkowska, adela adamus, and rafal baranski institute of plant biology and biotechnology, faculty of horticulture, university of agriculture in krakow, al. The protocols describe in detail all steps of dh production from donor plants growth conditions, through in vitro procedures. The best method to shortcut breeding technology is induction of haploid lines. Welcome to the how to use doubled haploids to improve.
Doubled haploid production for thousands of years, farmers have worked to improve their crop varieties, slowly breeding new traits over many generations. Esin ari1, tolga yildirim2, nedim mutlu1, saadet buyukalaca3, ummu gokmen4, ecehan akman1 1department of agricultural biotechnology, faculty of agriculture, akdeniz university, antalya, turkey. During the last years, our aim has been focused on the establishment of an efficient protocol for the production of doubled haploid plants from a wide range of genotypes, as well as from f1. As this trait is partly genetically controlled, the culture method has to be adapted to the plant genotype to re. It contains various prools and approaches of dh production proven for different germplasm of the same species. Production of doubled haploid wheat lines triticum aestivum. Nov, 20 doubled haploidy is a fundamental tool in plant breeding as it provides the fastest way to generate populations of meiotic recombinants in a genetically fixed state. Doubled haploid production in wheat through wide hybridization. The production of doubled haploids dh has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop. An isolated microspore culture provides an excellent system for the study of microspore induction and embryogenesis, provides a platform for an everincreasing array of molecular studies, and can produce doubled haploid dh plants, which are used to accelerate plantbreeding programs. Artificial production of doubled haploids is important in plant breeding. Haploid plants are characterized by possessing only a single set of chromosomes gametophytic number of chromosomes i. The production of doubled haploid dh plants has become a key tool in. Colchicine treatment concentration mgl colchicine treatment duration h number of regenerated plants number of doubled haploid plants 0 control 0 53 0 125 12 49.
Thus doubled haploid dh breeding reduces the time required for the development of new, truebreeding varieties by nearly an order of magnitude. However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also recently being applied in less advanced breeding. Overall purpose of dh production is to sample adequate genetic variation from the initial f 1 cross initial f 1. A simple method to improve efficiency of maize breeding cimmyt. The use of doubled haploids in onion breeding is limited due to the low gynogenesis efficiency of this species. Production and subsequent conversion of haploids into doubled haploids has many applications in genetics and plant breeding dunwell 2010.